Single-cell electrochemistry
Small-scale electrochemical methods have been used in our laboratories to monitor catecholamine, ascorbic acid, glucose, and oxygen dynamics at the single cell level. Electrochemistry with ultrasmall electrodes allows qualitative and quantitative analysis of single exocytosis (release) events and we are using these to develop an understanding of the molecular intricacies of these events. We are particularly interested in understanding the membrane mechanics driving vesicle opening, release of transmitter through the fusion pore, and the source of lipid allowing vesicles to contract and expand when treated pharmacologically. Part of this involves a collaboration with Christian Amatore at ENS in Paris. We are also working to understand the way in which lipid composition might affect or even regulate the rate of neurotransmitter release. Finally, we are also collaborating with Remco Westerink at Utrecht University to investigate the rapid effects of estrogen on exocytosis to determine the mechanism by which it is neuroprotective.
